Technology

Hi-C Proximity-Guided Assembly

Regain ultra-long-range genomic contiguity with Phase Genomics’ patent-pending Proximo™ Hi-C and ProxiMeta™ Hi-C technology.

chromosome-scale scaffolds for every genome

Get chromosome-scale scaffolds for virtually any genome

By approaching scaffolding in a fundamentally new way, Proximo Hi-C measures the proximity of DNA in vivo and uses that information to scaffold contigs into entire chromosomes. It only requires a biological sample in its natural, intact state — no need to isolate high-molecular weight DNA. These chromosome-scale scaffolds empower you to answer biological questions you never could before and to make important new discoveries.

By approaching scaffolding in a fundamentally new way, Proximo Hi-C measures the proximity of DNA in vivo and uses that information to scaffold contigs into entire chromosomes. It only requires a biological sample in its natural, intact state — no need to isolate high-molecular weight DNA. These chromosome-scale scaffolds empower you to answer biological questions you never could before and to make important new discoveries.

Crosslinked chromatin is fragmented and junctions are extracted. Fragmented junctions are proximity ligated and paired-end sequenced. Sequencing data encapsulates the chromatin proximity signal.

Promity data establishes relationships among contigs at ligation sites.

Contigs are placed into chromosome groups based on proximity signal...

...then ordered and oriented by proximity signal onto chromosome-scale scaffolds.

chromosome-scale scaffolds for every genome

Explore the depths of the microbial world

Our ProxiMeta metagenomic deconvolution method reveals which DNA sequences originated in the same cell, enabling assembly of whole genomes from mixed samples in their natural state — no culturing or high-molecular-weight DNA extraction required. Like Proximo Hi-C, ProxiMeta Hi-C is based on capturing physical DNA proximity with in vivo Hi-C. This powerful new source of information allows sequences from the same species and/or strain to be grouped, yielding dozens or even hundreds of genomes for rare, unculturable, and novel microbes easily and affordably.

In vivo crosslinking traps intra-cellular DNA contacts, including inter-chromosomal and plasmid-genome interactions. Because crosslinking is performed in vivo, inter-cellular interactions do not occur.

Crosslinked loci are fragmented and proximity ligated, creating chimeric junctions between sequences originating from the same cell. Paired-end sequencing these junctions yields proximity signal that can be used to group sequences by cellular origin.

Intracellular proximity signal is used to deconvolute metagenomes by grouping sequences into species- and strain-specific clusters. Multi-chromosome genomes can also be assembled, and plasmids can be assigned to host organisms.

Complete chromosome-scale scaffolds and deconvoluted metagenomes, along with a continuing commitment to personalized service without hidden costs.